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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
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1Institute of Sustainable Processes,University of Valladolid, 2Department of Chemical Engineering and Environmental Technology,University of Valladolid, 3Department of Public Health Microbiology,National Laboratory of Health, Environment and Food, 4ICBAS – School of Medicine and Biomedical Sciences,Porto University, 5Epidemiology Research Unit (EPIUnit),Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)
Chapters
- 00:04Introduction
- 01:00Wastewater and Air Samples Collection and Pre‐Processing
- 01:55Quantification of SARS‐CoV‐2 RNA by RT‐qPCR
- 02:46Sequencing the Variants
- 07:37Results: Detection and Quantification of SARS‐CoV‐2 RNA in Wastewater and Air Samples
- 08:31Conclusion
This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.
