Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and <em>S</em>-Genotype Analyses

Muhammad Husnain Ahmad; Xin Zheng; Yu Hu; Huimin Liu; Yi Sun; Hao Wen; Lijun Chai

doi:10.3791/65056

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Bestemmelse af selv(in)kompatibilitet og inter(in) kompatibilitetsforhold i citrus ved hjælp af manuel bestøvning, mikroskopi og S-genotypeanalyser

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Biology

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JoVE Journal Biology

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Bestemmelse af selv(in)kompatibilitet og inter(in) kompatibilitetsforhold i citrus ved hjælp af manuel bestøvning, mikroskopi og S-genotypeanalyser

DOI:

10.3791/65056-v

•

07:12 min

•

June 30, 2023

•

Muhammad Husnain Ahmad, Xin Zheng, Yu Hu, Huimin Liu, Yi Sun, Hao Wen, Lijun Chai

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,Huazhong Agricultural University

Chapters

00:05Introduction
01:02Pollen Collection
01:40In Vitro Pollen Germination Test
02:27Pollination
03:02Sampling, Fixation, and Preservation
03:55Aniline Blue Staining
04:46Fluorescence Microscopy
05:26Results: Determining Pollen Compatibility and Incompatibility in Citrus Cultivars
06:16Conclusion

Summary

Automatic Translation

Denne protokol giver en hurtig metode til bestemmelse af pollenkompatibilitet og uforenelighed i citrussorter.

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