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Human saphenøs veneendotelcelleisolering og eksponering for kontrollerede niveauer af forskydningsstress og strækning
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JoVE Journal Medicine
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human saphenøs veneendotelcelleisolering og eksponering for kontrollerede niveauer af forskydningsstress og strækning

DOI:
10.3791/65122-v

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09:10 min

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April 21, 2023

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, , , , , ,
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina,Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences,Carl von Ossietzky University of Oldenburg

Chapters

  • 00:04Introduction
  • 01:18Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
  • 03:37Applying Shear Stress on Human SVECs
  • 05:42Applying Cyclic Stretch on Human SVECs
  • 07:20Results: Effects of Mechanical Stress in Human SVECs
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en protokol til isolering og dyrkning af humane saphenøse veneendotelceller (hSVEC'er). Vi leverer også detaljerede metoder til at producere forskydningsspænding og stræk for at studere mekanisk belastning i hSVEC'er.

Tags

Keywords: Saphenous VeinEndothelial Cell IsolationShear StressStretchCoronary Artery Bypass SurgeryMechanical StressCell CultureCollagenaseSmooth Muscle CellsFibroblastsGrowth FactorsPBSCell MediumCentrifugationCell PelletGelatin CoatingCell Culture Dish

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