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Journal
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Biology
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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research (Video) | JoVE
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research (Video) | JoVE
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/65219-v
•
02:35 min
•
April 07, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Keywords: 3D Organoids
Intestinal Crypts
Intestinal Stem Cells
Homeostasis
Epithelial Growth
Differentiation
EDTA
Mechanical Dissociation
Signaling
Intestinal Epithelium
Paneth Cells
Enteroendocrine Cells
Enterocytes
Goblet Cells
Tuft Cells
Leucine-rich Repeats Containing G Protein-coupled Receptor 5
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