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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
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Generazione di linee cellulari knockout CENP-E-/- associate al centromero della proteina E utilizzando il sistema CRISPR/Cas9
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JoVE Journal Biology
Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
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Generazione di linee cellulari knockout CENP-E-/- associate al centromero della proteina E utilizzando il sistema CRISPR/Cas9

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:
10.3791/65476-v

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02:19 min

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June 23, 2023

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, , , , , , , ,
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, 4Medical Research Center,Fujian Maternity and Child Health Hospital

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Keywords: CENP-ECRISPR/Cas9KnockoutHeLa CellsChromosome AlignmentMitotic DefectsCell Cycle ArrestCell DeathKinetochore-microtubule CaptureSpindle Assembly Checkpoint

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