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1Molecular Surgery Laboratory,Stanford University, 2Department of Ophthalmology, Byers Eye Institute,Stanford University, 3Department of Ophthalmology and Visual Neurosciences,University of Minnesota, 4Retina Consultants of Minnesota, 5Department of Pediatrics,University of Iowa, 6Department of Neurology,University of Iowa, 7The Iowa Neuroscience Institute (INI),University of Iowa, 8Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary, 9Department of Biochemistry and Molecular Biology, Cumming School of Medicine,University of Calgary, 10Veterans Affairs Palo Alto Health Care System
Chapters
- 00:12Introduction
- 01:08Collection of aqueous humor liquid biopsies
- 02:54mCollection of vitreous liquid biopsies
- 06:56Transferring cryovials to storage
- 08:10Sample storage form
- 12:10Representative Results
- 14:19Conclusions
This protocol presents an integrated biorepository platform for the standardized collection, annotation, and biobanking of high-quality human aqueous humor and vitreous liquid biopsies for molecular downstream analyses, including proteomics, metabolomics, and glycomics.
Tags
Keywords:
BiobankingLiquid BiopsyAqueous HumorVitreousMolecular ProfilingOcular DiseaseDiagnosticTherapeuticStandardized CollectionData PrivacyBarcodeSample RetrievalProtein AnalysisGlycan AnalysisMetabolite AnalysisAnterior Chamber ParacentesisPhakic EyeVitrectomyVitreoretinal SurgeonUndiluted Sample
