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Journal
/
Cancer Research
/
Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen
/
Author Spotlight: Enhancing Nuclei Isolation for Multiome Sequencing in Challenging Tumor Microenvironments
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
Author Spotlight: Enhancing Nuclei Isolation for Multiome Sequencing in Challenging Tumor Microenvironments
Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen
DOI:
10.3791/65831-v
•
01:39 min
•
October 13, 2023
•
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
Tags
Keywords: Nuclei Isolation
Solid Tumor
Multiome Sequencing
10x Genomics
Pancreatic Ductal Adenocarcinoma
Cryopreservation
Nuclear Isolation
Cell Heterogeneity
Desmoplastic Matrix
Cell Yield
Cell Quality
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