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Journal
/
Cancer Research
/
Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen
/
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen
DOI:
10.3791/65831-v
•
01:39 min
•
October 13, 2023
•
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
Tags
Nuclei Isolation
Solid Tumor
Multiome Sequencing
10x Genomics
Pancreatic Ductal Adenocarcinoma
Cryopreservation
Nuclear Isolation
Cell Heterogeneity
Desmoplastic Matrix
Cell Yield
Cell Quality
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