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Journal
/
Biology
/
Two-Step Cell Digestion of Mouse Carotid Artery
/
Bereiding van een eencellige suspensie uit halsslagaders van muizen voor eencellige sequencing
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
Bereiding van een eencellige suspensie uit halsslagaders van muizen voor eencellige sequencing
Two-Step Cell Digestion of Mouse Carotid Artery
DOI:
10.3791/65863-v
•
01:16 min
•
January 19, 2024
•
Fengchan Li
,
Zhen Zhu
,
Yun Du
,
Li Zhu
2,3
,
Chaojun Tang
2,3
1
Cyrus Tang Hematology Center, Cyrus Tang Medical Institute
,
Soochow University
,
2
Collaborative Innovation Center of Hematology of Jiangsu Province
,
Soochow University
,
3
Suzhou Key Laboratory of Thrombosis and Vascular Biology
,
Soochow University
Tags
Single-cell Suspension
Mouse Carotid Arteries
Single-cell Sequencing
Carotid Stenosis
Carotid Atherosclerosis
Two-step Digestion Method
Collagenase/DNase
Trypsin
Cell Viability
Cell Concentration
Vascular Smooth Muscle Cells (VSMCs)
Fibroblasts
Endothelial Cells (ECs)
Macrophages And Dendritic Cells (M&DCs)
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Bereiding van een eencellige suspensie uit halsslagaders van muizen voor eencellige sequencing
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