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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

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Hücre Döngüsünün G1 Fazında Tek Sarmallı DNA Odaklarının Görselleştirilmesi

JoVE Journal
Biology

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JoVE Journal Biology

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Please note that all translations are automatically generated. Click here for the English version.

Hücre Döngüsünün G1 Fazında Tek Sarmallı DNA Odaklarının Görselleştirilmesi

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

DOI:

10.3791/65926-v

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01:21 min

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December 22, 2023

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Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

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Keywords: Single-stranded DNA DNA Damage Response DNA Repair RPA2 Cell Cycle G1 Phase Immunofluorescence DNA Lesions DNA Polymerases SsDNA Foci

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