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Journal
/
Neuroscience
/
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
/
Enriquecimento rápido e eficiente da microglia medular de camundongos
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia
Enriquecimento rápido e eficiente da microglia medular de camundongos
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
DOI:
10.3791/65961-v
•
01:52 min
•
September 22, 2023
•
Claudia I Gutiérrez-Román
2
,
María E Meléndez Camargo
,
Cuauhtémoc C García Rojas
,
Miguel Jimenez Olvera
,
Sandra H Gutiérrez Román
,
Oscar Medina-Contreras
1
Epidemiology, Endocrinology & Nutrition
,
Mexico Children’s Hospital
,
2
Pharmacy Department, National School of Biological Sciences
,
National Polytechnic Institute
,
3
Graduate Department
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
4
Pain Clinic and Palliative Care
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
5
General Surgery Service
,
Metropolitan Angeles Hospital
Tags
Keywords: Spinal Cord
Microglia
Purification
Isolation
Flow Cytometry
CD11b
CD45
Collagenase
Density Gradient
Heterogeneity
Central Nervous System
Neurological Pathologies
Chronic Pain
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