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Journal
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Developmental Biology
/
Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
/
Imagem em Tempo Real da Dinâmica do Cálcio Acrossomal e Exocitose em Espermatozoides Vivos de Camundongos
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm
Imagem em Tempo Real da Dinâmica do Cálcio Acrossomal e Exocitose em Espermatozoides Vivos de Camundongos
Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
DOI:
10.3791/65962-v
•
00:46 min
•
October 13, 2023
•
Roy Cohen
2
,
Danielle M. Sosnicki
,
Melissa A. White
,
Jacquelyn L. Nelson
,
Chinatsu Mukai
,
Alexander J. Travis
2
1
Baker Institute for Animal Health, College of Veterinary Medicine
,
Cornell University
,
2
Department of Public and Ecosystem Health, College of Veterinary Medicine
,
Cornell University
Tags
Real-time Imaging
Acrosomal Calcium Dynamics
Acrosome Exocytosis
Sperm
Calcium Signaling
Capacitation
Fertilization
AcroSensE
Genetically Encoded Calcium Indicator
GCaMP
MCherry
High Frame-rate Imaging
Stimulant Delivery System
Genetic Tools
CRISPR
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