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Suspension kultur: produktion og oprensning af adeno-associeret virus ved iodixanol densitetsgradient centrifugering til in vivo applikationer
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JoVE Journal Medicine
Suspension Culture Production and Purification of Adeno-Associated Virus by Iodixanol Density Gradient Centrifugation for In Vivo Applications

Suspension kultur: produktion og oprensning af adeno-associeret virus ved iodixanol densitetsgradient centrifugering til in vivo applikationer

AAV Isolation by Double Iodixanol Density Gradient Centrifugation

DOI:
10.3791/66460-v

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01:19 min

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February 09, 2024

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, ,
1Department of Pediatrics,University of Florida

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Keywords: Adeno-associated VirusAAVGene TherapyIodixanol Density Gradient CentrifugationRecombinant AAVSuspension CultureExpi293 CellsPEG PrecipitationBuffer ExchangeVirus PurificationVirus ProductionVirus YieldIn Vivo Applications

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