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Journal
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Bioengineering
/
Efficient Adeno-Associated Virus Vector Production Using a Cell Factory Platform
/
Un procedimiento simplificado y estandarizado para generar vectores de virus adenoasociados de alta calidad y alto título utilizando una plataforma de fábrica de células
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform
Please note that all translations are automatically generated.
Click here for the English version.
Un procedimiento simplificado y estandarizado para generar vectores de virus adenoasociados de alta calidad y alto título utilizando una plataforma de fábrica de células
Efficient Adeno-Associated Virus Vector Production Using a Cell Factory Platform
DOI:
10.3791/66741-v
•
01:52 min
•
May 03, 2024
•
Ting Zhang
,
Vinitha Dhamotharan
,
Yu Xiao
2
,
Sydne Ballengee
3
,
Jill Pollon
4
,
George K. Gittes
1
Division of Pediatric Surgery, Department of Surgery, Children’s Hospital of Pittsburgh
,
University of Pittsburgh School of Medicine
,
2
North Allegheny High School
,
3
Wright State University Boonshoft School of Medicine
,
4
College of Engineering
,
University of Michigan
Tags
Adeno-associated Virus
AAV Vectors
Cell Factory Platform
Cell Stacks
HEK293T Cells
Purification
Yield
Purity
Polyethylene Glycol
Iodixanol Gradient
SDS-PAGE
Transmission Electron Microscopy
Gene Therapy Research
Preclinical Studies
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Un procedimiento simplificado y estandarizado para generar vectores de virus adenoasociados de alta calidad y alto título utilizando una plataforma de fábrica de células
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