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Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

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Biology

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JoVE Journal Biology

Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

DOI:

•

07:23 min

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March 05, 2008

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Chi-Shuo Chen, Jonathan Pegan, Jesus Luna, Bing Xia, Kara McCloskey, Wei-chun Chin, Michelle Khine

¹School of Engineering,University of California Merced – UC Merced

Chapters

00:00Title
01:31Making Shrinky-Dink Mold
02:57Making PDMS Microwells
05:05Trapping Cells in Microwells
06:43Conclusion

Summary

Automatic Translation

We show a simple and rapid method to load pre-defined numbers of cells into microfabricated wells and maintain them for embryoid body development.

Tags

Shrinky-Dink Hanging Drops Simple Way Form And Culture Embryoid Bodies Embryonic Stem Cells Hanging Drop Method Pipetting Aggregates Stem Cell Differentiation Microfabricated Wells Red Fluorescent Mouse Cell Line

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