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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
JoVE Journal
Biology
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JoVE Journal Biology
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
DOI:

08:51 min

May 27, 2008

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Chapters

  • 00:18Introduction
  • 00:38X-ray radiation tomographic measurements
  • 02:08Removing the background and extracting the sample information
  • 02:38Rotating the object using the keyframer
  • 03:25Setting the virtual plane
  • 04:06Moving the slice through the object using the keyframer
  • 04:45User specific camera paths
  • 05:23Following the digestive system through the whole animal

Summary

Automatic Translation

We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.

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