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Counting and Determining the Viability of Cultured Cells
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JoVE Journal Biology
Counting and Determining the Viability of Cultured Cells

Counting and Determining the Viability of Cultured Cells

DOI:
10.3791/752-v

•

05:47 min

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June 23, 2008

•

,
1Molecular Pathology Laboratory Network, Inc

Chapters

  • 00:00Title
  • 00:36Introduction
  • 00:57Preparing the hemacytometer
  • 01:31Preparing cell suspension
  • 02:08Loading hemacytometer
  • 02:42Counting and calculating number of cells
  • 04:24Determination of cell viability
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.

Tags

CountingDeterminingViabilityCultured CellsStandardizationCulture ConditionsQuantitation ExperimentsHemacytometerGlass SlideCounting ChamberCell SuspensionCell Density Calculation

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