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Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus
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Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus

Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus

DOI:
10.3791/819-v

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14:05 min

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June 17, 2008

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, , , ,
1The University of Hong Kong – HKU

Chapters

  • 00:00Title
  • 00:10Introduction
  • 01:07Tools and Reagents
  • 02:12Procedures
  • 13:26Conclusion

Summary

Automatic Translation

Automatic Translation

This video describes the method of retrograde labeling of RGC by applying fluoro-gold (FG) on the surface of superior colliculus (SC). Technique involves drilling the skull, aspirating the cortex, and applying gelatin sponge over entire dorsal surface of SC.

Tags

Retrograde LabelingRetinal Ganglion CellsFluoro-goldSuperior ColliculusGlaucomaRGC CountingTreatment EvaluationRatAxon TerminalsSomasRetrogradely TransportedTransected Optic NerveRGC Survival InterferenceCresyl Violet StainingAmacrine CellsEndothelium Of Blood VesselSurgical ProceduresDrilling The SkullAspirating The CortexGelatin SpongeIntracranial Bleeding

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