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从MEFs基质胶2：到基质胶从MEFs分裂的胚胎干细胞
JoVE Journal
Biology
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JoVE Journal
Biology
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
Please note that all translations are automatically generated.
Click here for the English version.
从MEFs基质胶2：到基质胶从MEFs分裂的胚胎干细胞
DOI:
10.3791/831-v
•
06:40 min
•
June 09, 2008
•
Ivan Khvorostov
,
Jin Zhang
,
Michael Teitell
1
David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:00
Introduction
00:26
hESC Culture Daily Maintenance
01:50
Splitting hESCs onto Matrigel
06:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
本视频演示了如何维持人类胚胎干细胞（胚胎干细胞）在馈线无细胞的条件和如何在馈线不断通过人类胚胎干细胞的无细胞条件的增长。在馈线无细胞的条件下，免疫荧光显微镜确认的人类胚胎干细胞的全能性增长也表明。
Tags
MEFs
Matrigel
HESCs
Feeder Cells
Passage
Grow
Human Embryonic Stem Cells
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