Journal
/
Biology
/
The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

DOI:
10.3791/845-v

•

23:06 min

•

August 11, 2008

•

, , ,
1The Warren Alpert Medical School of Brown University, 2Laboratorio de Investigacion de Enfermedades Infecciosas,Universidad Peruana Cayetano Heredia, 3Department of International Health,Johns Hopkins Bloomberg School of Public Health, 4Wellcome Trust Centre for Clinical Tropical Medicine,Imperial College London

Chapters

  • 00:13Introduction
  • 03:18Equipment
  • 04:53Reagents
  • 06:20Stock solutions
  • 09:18Working solutions
  • 10:37Antibiotic working solutions
  • 11:19Decontamination
  • 13:15Adding antibiotics to samples
  • 14:19Quality control
  • 16:18Plate reading
  • 19:01Biosafety

Summary

Automatic Translation

Automatic Translation

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture method.

Tags

MODS MethodDiagnosisTuberculosisMultidrug Resistant TuberculosisActive Pulmonary TuberculosisDrug ResistanceMicroscopic-observation Drug-susceptibility AssayLiquid MediaSolid MediaMycobacterium TuberculosisAtypical MycobacteriaFungal ContaminationBacterial ContaminationIsoniazidRifampicinDirect DetectionSubcultureSensitivitySputum SmearCulture Methods

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/845?article_type=v

Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
check_url/845?article_type=v

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
check_url/845?article_type=v

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
check_url/845?article_type=v

En PCR-baseret genotypebestemmelse metode til at skelne mellem vildtype og Ornamental sorter af Imperata cylindrica</em
check_url/845?article_type=v

High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode
check_url/845?article_type=v

En enkel metode til Imaging Arabidopsis Blade Brug Perfluorodecalin som en infiltrativ Imaging Medium
check_url/845?article_type=v

Tomat / GFP-FLP / FRT Metode til Live Imaging af Mosaic Voksen<em> Drosophila</em> fotoreceptorceller
check_url/845?article_type=v

Fremgangsmåde til screening og validering af resistente mutationer Against kinaseinhibitorer
check_url/845?article_type=v

Metode til vurdering af effekter af en række bølgelængder og intensiteter af Rød / nær-infrarødt lys terapi på oxidativt stress<em> In vitro</em
check_url/845?article_type=v

Filtrering Isolering af nukleinsyrer: en enkel og hurtig DNA Extraction Method

Read Article