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Counting Human Neural Stem Cells
JoVE Journal
Biology
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JoVE Journal Biology
Counting Human Neural Stem Cells
DOI:

06:37 min

August 22, 2007

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Chapters

  • 00:00Title
  • 00:12Passaging Human Embryonic Neural Stem Cells
  • 00:30Preparing the Cell Suspension for Counting
  • 03:54Counting Cells in the Hemocytometer

Summary

Automatic Translation

Knowledge of the exact number of viable cells is required for many tissue culture manipulations. This protocol describes how to differentiate between live and dead cells and quantify cells using a hemacytometer. Although it describes counting human neural stem/precursor cells (hNSPCs), it can be used for other cell types.

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