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Chapters
- 00:05Title
- 01:36Staining Nematodes with DiI
- 04:24Mounting DiI-stained Nematodes
- 05:40DiI Staining of C. elegans Cuticle
- 07:52Conclusion
We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.
Tags
VisualizationCaenorhabditis ElegansCuticular StructuresLipophilic Vital Dye DiICuticleResistant StructureEnvironment ProtectionBody ShapeMotilityEpidermal CellsLipid LayerCircumferential RidgesAnnuliAlaeDevelopment StagesMutationsCollagen OrganizationCompound MicroscopyDIC OpticsFluorescent Transgene ExpressionAntibody StainingElectron MicroscopyWheat Germ Agglutinin (WGA)Cuticular GlycoproteinsFluorescent DyeLive Imaging
