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Journal / Biology / Isolating Intestinal Stem Cells from Adult…
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JoVE Journal Biology
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
 

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Article DOI: 10.3791/52223-v • 10:57 min • December 16th, 2014
December 16th, 2014 •
*1, *2, 1
1Institut für Biochemie und Molekulare Biologie, Universität Ulm, 2Institut für Immunologie, Universitätsklinikum Ulm
* These authors contributed equally

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Summary
Automatic Translation

Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

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Keywords: Intestinal Stem Cells Drosophila FACS Aging Midgut Enteroblast GFP Cell Isolation Tissue Homeostasis
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