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Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
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Immunology and Infection
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JoVE Journal Immunology and Infection
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

DOI:
10.3791/54242-v

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08:41 min

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June 07, 2017

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,
1Department of Medicine,Icahn School of Medicine at Mount Sinai, 2Immunología de Trasplantes, Centro Nacional de Microbiología,Instituto de Salud Carlos III

Chapters

  • 00:05Title
  • 00:45Graft-infiltrating Macrofage Isolation by Fluorescence-activated Cell Sorting (FACS)
  • 03:23T Cell Isolation by FACS
  • 06:39Results: Representative In Vitro Macrophage Subset Suppressive Capacity Analyses
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Macrophages are plastic cells of the hematopoietic system that have a crucial role in protective immunity and homeostasis. In this report, we describe optimized in vitro techniques to phenotypically and functionally characterize graft-infiltrating regulatory macrophages that accumulate in the transplanted organ under tolerogenic conditions.

Tags

Keywords: MacrophagesRegulatory MacrophagesGraft-reactive ImmunityAllograftSingle-cell SuspensionFlow CytometryCell SortingCD11bCD45Ly6CLy6GDAPIViabilityCell Culture

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