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Mass Histology to Quantify Neurodegeneration in Drosophila
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JoVE Journal Neuroscience
Mass Histology to Quantify Neurodegeneration in Drosophila

Mass Histology to Quantify Neurodegeneration in Drosophila

DOI:
10.3791/54809-v

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06:34 min

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December 15, 2016

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,
1Oregon Institute of Occupational Health Sciences,Oregon Health & Sciences University

Chapters

  • 00:05Title
  • 00:58Fixing and Paraffin-embedding Drosophilia Heads
  • 02:36Sectioning, Mounting, and Microscopy
  • 04:38Results: Mass Histology Allows for Large-scale Analysis of Neurodegeneation in Drosophila
  • 05:59Conclusion

Summary

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Automatic Translation

Drosophila is widely used as a model system to study neurodegeneration. This protocol describes a method by which degeneration, as determined by vacuole formation in the brain, can be quantified. It also minimizes effects due to the experimental procedure by processing and sectioning control and experimental flies as one sample.

Tags

Mass HistologyNeurodegenerationDrosophilaBrainNeurodegenerative DiseasesGenetic ModificationsEnvironmental InfluencesStainingForcepsCollarsEyeless Senoculus FliesCarnoy’s SolutionEthanolMethyl BenzoateParaffinEmbeddingParaffin BlocksSectioningHeating PlateRazor BladesMounting Blocks

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