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Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
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Immunology and Infection
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JoVE Journal Immunology and Infection
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

DOI:
10.3791/55800-v

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05:31 min

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August 07, 2017

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, , , , , ,
1Copenhagen Prospective Studies on Asthma in Childhood (COPSAC), Herlev and Gentofte Hospital,University of Copenhagen, 2Department of Biotechnology and Biomedicine,Technical University of Denmark

Chapters

  • 00:05Title
  • 00:42Patient Sampling
  • 01:42Airway Immune Mediator Quantification
  • 03:18Results: Representative Upper Airway Immune-mediator Analyses
  • 04:56Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a noninvasive technique for the sampling of undisturbed mucosal lining fluid from the upper airways. It can be used to perform the quantification of in vivo levels of protein mediators, such as cytokines and chemokines, in subjects of all ages.

Tags

Noninvasive SamplingMucosal Lining FluidAirway Immune MediatorsPediatric Respiratory DiseasesFilter PaperNasal ExtractImmune Mediator QuantificationElectrochemiluminescence-based Assay

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