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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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Chapters
- 00:04Title
- 00:31Sample Preparation
- 01:40Atomic Force Microscope (AFM) Setup
- 03:27AFM Calibration and Data Acquisition
- 05:28Results: AFM-Based Single-Molecule Force Spectroscopy of (I91)3-NI10C-(I91)3
- 06:20Conclusion
We describe the detailed procedures and strategies to measure the mechanical properties and mechanical unfolding pathways of single protein molecules using an atomic force microscope. We also show representative results as a reference for selection and justification of good single protein molecule recordings.
