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Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
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JoVE Journal Biology
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

DOI:
10.3791/56030-v

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08:04 min

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July 14, 2017

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, , , , , ,
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University

Chapters

  • 00:05Title
  • 00:55Isolation of Sialic Acids from Milk and from Liver Tissue
  • 03:26Preparation of a Mixed Sialic Acid Standard
  • 04:26Fluorescence Derivatization of Sialic Acids
  • 05:18HPLC Analysis of Sialic Acid Derivatives
  • 06:15Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.

Tags

Sialic AcidN-Acetylneuraminic AcidN-Glycolylneuraminic AcidCMAHLiverMilkHPLCGlycoscienceMouse ModelSample PreparationAnion Exchange Chromatography

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