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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

DOI:
10.3791/56132-v

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10:17 min

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October 05, 2017

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,
1Institute for Environmental Sciences,University of Koblenz-Landau, 2Institute of Integrated Natural Sciences,University of Koblenz-Landau, 3IV 2.5 (Trace Analysis, Artificial Pond and stream system),Federal Environment Agency

Chapters

  • 00:05Title
  • 01:01Macroinvertebrate Sample Preparation for Genetic Gut Content Analysis
  • 03:56Verification of Functional Efficiency of DNA Extracts
  • 05:37Automated Fragment Analysis for Parallel Detection of Multiple, Recently Ingested Prey Groups
  • 08:12Results: Predator Diet Analyses Using Group-specific Prey DNA Primers
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.

Tags

Keywords: Genetic Gut Content AnalysisAquatic MacroinvertebratesRDNA PrimersTrophic EcologyFood WebInvasive SpeciesDNA ExtractionProteinase KSDSSodium ChlorideIsopropanolPCRPrimer Design

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