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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

DOI:
10.3791/56386-v

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09:57 min

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March 05, 2018

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, , , , , , , , , , , , , , , , ,
1Department of Hematology,VU University Medical Center, 2Pediatric Oncology/Hematology,VU University Medical Center, 3Janssen Research & Development, LLC, 4Princess Máxima Center for Pediatric Oncology

Chapters

  • 00:05Title
  • 01:06Bone Marrow Aspiration and Smear Preparation
  • 03:16Flow Cytometry Setup
  • 05:53Cell Staining for Flow Cytometry
  • 07:45Results: Representative Leukemia-associated Immunophenotype (LAIP) Gating
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

Detection of minimal or measurable residual disease (MRD) is an important prognostic biomarker for refining risk assessment and predicting relapse in acute myeloid leukemia (AML). These comprehensive guidelines and recommendations with best practices for consistent and accurate identification and detection of MRD, may aid in making effective AML treatment decisions.

Tags

Bone MarrowAcute Myeloid LeukemiaMeasurable Residual DiseaseLeukemia Stem CellsSamplingProcessingProtocolHematologyDiagnosisTreatment Monitoring

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