Journal
/
Immunology and Infection
/
Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

DOI:
10.3791/57171-v

•

08:24 min

•

February 27, 2018

•

, , , , , ,
1Department of Biology,Middle Tennessee State University, 2DRVision Technologies

Chapters

  • 00:04Title
  • 00:50Capsule Induction and Yeast Cell Staining
  • 03:14Image Acquisition
  • 04:27Capsule Measurement
  • 06:33Results: Representative Capsule Measurement
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.

Tags

Cryptococcus NeoformansCapsule DiameterCapsule StainingIndia InkCalcofluor-whiteAlexa Fluor 488Capsule MeasurementVirulenceAutomated Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57171?article_type=v

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
check_url/57171?article_type=v

Measurement of γHV68 Infection in Mice
check_url/57171?article_type=v

Quantitative Measurement of the Immune Response and Sleep in Drosophila
check_url/57171?article_type=v

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis
check_url/57171?article_type=v

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
check_url/57171?article_type=v

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
check_url/57171?article_type=v

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
check_url/57171?article_type=v

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
check_url/57171?article_type=v

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
check_url/57171?article_type=v

A Method for the Measurement of Salivary Gland Function in Mice

Read Article