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The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
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Chapters
- 00:04Title
- 00:51Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
- 04:10Culture of EPDCs
- 05:32Induction of Epithelial to Mesenchymal Transition in EPDCs
- 06:31Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
- 08:17Conclusion
The epicardium plays a crucial role in the development and repair of the heart by providing cells and growth factors to the myocardial wall. Here, we describe a method to culture human primary epicardial cells that enables the study and comparison of their developmental and adult characteristics.
