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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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Genetics
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JoVE Journal Genetics
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

DOI:
10.3791/58089-v

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07:56 min

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November 16, 2018

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, , , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, 2Department Life,Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology,Rostock University Medical Center

Chapters

  • 00:04Title
  • 00:33Isolation of hDFSCs
  • 02:50Transfection of hDFSCs
  • 04:07Analysis of Transfection
  • 06:02Results: Successful Isolation and Genetic Modification of hDFSC
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the transient genetic engineering of dental stem cells extracted from the human dental follicle. The applied non-viral modification strategy may become a basis for the improvement of therapeutic stem cell products.

Tags

Keywords: Dental Follicle Stem CellsHDFSCsEnzymatic DigestionCollagenaseDispaseMicroRNAGenetic ModificationRegenerative MedicineCell IsolationCell Characterization

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