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Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model
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Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model

Targeted and Selective Treatment of Pluripotent Stem Cell-derived Teratomas Using External Beam Radiation in a Small-animal Model

DOI:
10.3791/58115-v

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05:08 min

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February 17, 2019

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, , , , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Department of Medicine, Division of Cardiology,Stanford University School of Medicine, 3Medical Service, Cardiology Section,Veteran Affairs Palo Alto Health Care System, 4Department of Pathology,Stanford University School of Medicine, 5Department of Radiology, Molecular Imaging Program,Stanford University School of Medicine, 6Peking University Shenzhen Health Science Institute, 7Department of Radiation Oncology,Stanford University School of Medicine

Chapters

  • 00:04Title
  • 00:57Pluripotent Stem Cell (PSC) Transplantation, Bioluminescence Imaging (BLI), and Teratoma Irradiation
  • 03:48Results: Representative Successful Tumor Induction and Treatment
  • 04:25Conclusion

Summary

Automatic Translation

Automatic Translation

Research on treatment strategies for pluripotent stem cell-derived teratomas is important for the clinical translation of stem cell therapy. Here, we describe a protocol to, first, generate stem cell-derived teratomas in mice and, then, to selectively target and treat these tumors in vivo using a small-animal irradiator.

Tags

Keywords: Stem CellTeratomaExternal Beam RadiationSmall Animal ModelIPSCsBioluminescence ImagingMatrigelAthymic Nude MiceCell TherapyTargeted Treatment

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