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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
 

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Article DOI: 10.3791/58431-v • 07:39 min • January 7th, 2019
January 7th, 2019 •
1, 1, 2, 1, 1
1Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute, University of Leicester, 2MRC Toxicology Unit, University of Leicester

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Summary
Automatic Translation

Here, a protocol for the isolation and characterization of CD4+ T-cell subsets from human peripheral blood is described. Purified CD4+ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.

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Keywords: CD4+ T-cells T-follicular Helper (Tfh) Cells Circulating T-follicular Helper (cTfh) Cells Flow Cytometry Biomarker Autoimmune Disease B-cell Non-Hodgkin's Lymphoma Peripheral Blood Cell Isolation Cell Subsets
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