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Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography
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JoVE Journal Biology
Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

DOI:
10.3791/58501-v

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06:21 min

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October 09, 2018

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, , , , ,
1Beijing Key Lab of Digital Plant, Beijing Research Center for Information Technology in Agriculture,Beijing Academy of Agriculture and Forestry Sciences

Chapters

  • 00:04Title
  • 00:52Sample Preparation Protocol
  • 02:19Micro-CT Scanning Protocol
  • 03:03“Image Analysis for a Single CT Imageof a Maize Stem or Leaf”
  • 04:06Image Analysis Protocol for CT Image Series of a Maize Root
  • 05:09Results: Phenotypic Traits of Vascular Bundles in Maize Stems, Leaves, and Roots Computed
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a novel method to improve the X-ray absorption contrast of maize tissue suitable for ordinary microcomputed tomography scanning. Based on CT images, we introduce a set of image-processing workflows for different maize materials to effectively extract microscopic phenotypes of vascular bundles of maize.

Tags

Micron-scale PhenotypingMaize Vascular BundlesX-ray Microcomputed TomographySample PreparationCT ScanningPhenotypic AnalysisPlant MaterialsFAA SolutionEthanol DehydrationCritical Point DryingIodine StainingScanning ParametersImage ReconstructionAutomatic Imaging Software

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