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Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi
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JoVE Journal Genetics
Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi

Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi

DOI:
10.3791/58521-v

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06:44 min

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October 05, 2018

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,
1Center for Bioscience Research and Education,Utsunomiya University

Chapters

  • 00:04Title
  • 00:39Field Collection and Maintenance of V. emeryi Colonies in the Laboratory
  • 01:31Experimental Laboratory Crosses
  • 03:08DNA Extraction of the F0 Generation
  • 04:08DNA Extraction of the F1 Generation
  • 05:46Results: Inbred Male V. emeryi Sterile
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, methods for conducting inbreeding crosses, and for evaluating the success of those crosses, are described for the ant Vollenhovia emeryi. These protocols are important for experiments aimed at understanding the genetic basis of sex determination systems in Hymenoptera.

Tags

InbreedingVollenhovia EmeryiAntSex DeterminationGenetic MappingMolecular BiologyHymenopteraColony MaintenanceEnvironmental ConditionsExperimental CrossingEgg Observation

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