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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

DOI:
10.3791/58664-v

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07:07 min

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January 07, 2019

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1Pulmonary, Immunology and Physiology Laboratory, Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State University College of Medicine

Chapters

  • 00:04Title
  • 00:31Estrous Cycle Stage Assessment, Ozone Exposure, and Lung Collection
  • 02:11RNA Preparation
  • 03:43microRNA (miRNA) Profiling
  • 04:40Results: Representative Functional microRNA Analyses
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method to assess lung expression of miRNAs that are predicted to regulate inflammatory genes using mice exposed to ozone or filtered air at different stages of the estrous cycle.

Tags

MicroRNALungEstrous CycleOzoneMouseInflammationVaginal FluidRNA ExtractionReal-time PCR

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