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JoVE Journal Cancer Research
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
 

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Article DOI: 10.3791/58710-v • 11:05 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1, 2, 3, 1
1Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute, 2Blood Research Institute, Blood Center of Wisconsin, 3Department of Bioengineering, University of California, San Diego

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Summary
Automatic Translation

This manuscript describes a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR-Cas9-based method for simple and expeditious investigation of the role of multiple candidate genes in Acute Myeloid Leukemia (AML) cell proliferation in parallel. This technique is scalable and can be applied in other cancer cell lines as well.

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Keywords: CRISPR-Cas9 Competition Assays Genetic Dependencies AML Single Guide RNA Oligonucleotides Annealing Ligation Vector Linearization High-throughput Screening
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