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Journal / Immunology and Infection / Interrogating Individual Autoreactive Germinal…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
 

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Article DOI: 10.3791/59397-v • 11:12 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1
1Department of Biomedicine, Aarhus University

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Summary
Automatic Translation

This protocol describes the generation of mixed murine bone marrow chimeras with spontaneous autoimmune germinal centers, in which autoreactive lymphocytes carry a photoactivatable green fluorescent protein (PA-GFP) reporter. This provides the ability to link cellular location in tissues with downstream molecular and functional analyses.

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Keywords: Autoimmunity Autoreactive Germinal Centers Mixed Chimeric Model Photoactivation Bone Marrow Chimera Immunology Immuno-oncology Stem Cell Research Bone Marrow Extraction Bone Marrow Reconstitution
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