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Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
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JoVE Journal Genetics
Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

DOI:
10.3791/59422-v

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06:57 min

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June 14, 2019

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, , , , , , , , ,
1Department of Pathology, Georges Pompidou European Hospital, Assistance publique – Hôpitaux de Paris (APHP),Paris Descartes University, 2Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, 3Laboratory of Immunology, Georges Pompidou European Hospital, APHP,Paris Descartes University

Chapters

  • 00:04Title
  • 00:49Preparation of 1x Target Retrieval Reagent
  • 01:22Tissue Pretreatment
  • 03:12Hybridization
  • 04:49Signal Detection with 3,3’-diaminobenzidine
  • 05:45Results: “Low” and “High” RNA CISH Staining
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Human papillomavirus (HPV) RNA chromogenic in situ hybridization is considered to be one of the gold standards for active human papillomavirus infection detection within tumors. It allows the visualization of HPV E6-E7 mRNA expression with localization and semiquantitative evaluation of its signal.

Tags

Keywords: Chromogenic In Situ HybridizationHPV RNA CISHHPV-related Head And Neck CancerActive HPV InfectionOropharyngeal Squamous Cell CarcinomaCervical NeoplasiaTarget Retrieval ReagentHydrogen PeroxideProtease DigestionHybridization Oven

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