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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
DOI:

10:01 min

April 08, 2020

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Chapters

  • 00:05Introduction
  • 00:45Protein Expression and Preparation
  • 03:38Dye-modification of Elastin-like Proteins via SPAAC
  • 04:44THF Swelling Protocol
  • 06:29BuOH Extrusion Protocol
  • 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
  • 09:22Conclusion

Summary

Automatic Translation

At the interface of organic and aqueous solvents, tailored amphiphilic elastin-like proteins assemble into complex supramolecular structures such as vesicles, fibers and coacervates triggered by environmental parameters. The described assembly protocols yield Protein Membrane-Based Compartments (PMBCs) with tunable properties, enabling the encapsulation of various cargo.

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