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Immunology and Infection
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In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

DOI:
10.3791/61068-v

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09:19 min

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May 24, 2020

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, , , , , ,
1Department of Pulmonary Diseases, Amsterdam UMC,VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group,University of Twente, 3Applied Stem Cell Technologies Group,University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC,VU University Medical Center, 5Institute of Physiology,Charité-Universitätsmedizin, 6German Heart Center

Chapters

  • 00:04Introduction
  • 00:59Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
  • 02:43Flow Chambers and PAEC Monolayer Preparation
  • 04:00Washed Human Whole Blood Preparation
  • 04:50Flow System Assembly
  • 05:41Washed Whole Blood Perfusion
  • 06:44Results: Representative Thrombus Formation Characterization
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation.

Tags

In VitroMicrofluidicDisease ModelWhole BloodEndothelialBlood ClotThrombogenicityAnticoagulantInflammatoryPersonalized MedicinePulmonary ArteryFibroronectinEndothelial CellCD144Flow CytometryFlow ChamberGelatin

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