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Immunology and Infection
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Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

DOI:
10.3791/61180-v

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09:16 min

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June 18, 2020

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, , ,
1Department of Medicine,Monash University, 2Monash Micro Imaging,Monash University, 3Immunology Department,Monash Health

Chapters

  • 00:04Introduction
  • 01:00Extracellular DNA (ecDNA) Staining
  • 03:50Staining Analysis
  • 07:18Results: Representative ecDNA Identification and Quantification
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.

Tags

Supervised Machine LearningSemi-quantificationExtracellular DNAGlomerulonephritisKidney HistologyAntigen RetrievalImmunofluorescence StainingSudan Black Quenching

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