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Biochemistry

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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
 

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Summary
Automatic Translation

This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

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Keywords: Subcellular Compartment Redox-sensitive GFP Oxidative Stress Redox State Ratiometric Non-disruptive Transduction Flow Cytometry Hydrogen Peroxide
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