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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
 

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Article DOI: 10.3791/62040-v • 07:38 min • April 30th, 2021
April 30th, 2021 •
1,2, 1, 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

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Summary
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The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Keywords: Pacemaker Regions Renal Pelvis Vibratome Sectioning Intact Preparation Smooth Muscle Kidney Ureter Calcium Ion Imaging Dissection Tissue Forceps Razor Blade KRB Solution
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