Manfred Auer Department of Biomedical Engineering, School of Biological Sciences and Medical Engineering Southeast University Biography Publications Institution JoVE Articles Manfred Auer has not added a biography. If you are Manfred Auer and would like to personalize this page please email our Author Liaison for assistance. Publications Electron Tomography Unravels New Insights into Fiber Cell Wall Nanostructure; Exploring 3D Macromolecular Biopolymeric Nano-architecture of Spruce Fiber Secondary Walls Scientific Reports. Feb, 2023 | Pubmed ID: 36759530 Temporal Dynamics of Fibrolytic and Methanogenic Rumen Microorganisms During in Situ Incubation of Switchgrass Determined by 16S RRNA Gene Profiling Frontiers in Microbiology. 2014 | Pubmed ID: 25101058 Cellulosic Biomass Pretreatment and Sugar Yields As a Function of Biomass Particle Size PloS One. 2014 | Pubmed ID: 24971883 Death and Transfiguration in Static Staphylococcus Epidermidis Cultures PloS One. 2014 | Pubmed ID: 24964210 A Loss of Function Analysis of Host Factors Influencing Vaccinia Virus Replication by RNA Interference PloS One. 2014 | Pubmed ID: 24901222 Novel 1:1 Labeling and Purification Process for C-terminal Thioester and Single Cysteine Recombinant Proteins Using Generic Peptidic Toolbox Reagents Bioconjugate Chemistry. Jul, 2014 | Pubmed ID: 24866260 Identification and X-ray Co-crystal Structure of a Small-molecule Activator of LFA-1-ICAM-1 Binding Angewandte Chemie (International Ed. in English). Apr, 2014 | Pubmed ID: 24692345 Exploring the Role of CheA3 in Desulfovibrio Vulgaris Hildenborough Motility Frontiers in Microbiology. 2014 | Pubmed ID: 24639670 Twist1-induced Dissemination Preserves Epithelial Identity and Requires E-cadherin The Journal of Cell Biology. Mar, 2014 | Pubmed ID: 24590176 Biomimetic Virus-based Colourimetric Sensors Nature Communications. 2014 | Pubmed ID: 24448217 High-throughput Chemical Screening for Antivirulence Developmental Phenotypes in Trypanosoma Brucei Eukaryotic Cell. Mar, 2014 | Pubmed ID: 24442893 ACF7 is a Hair-bundle Antecedent, Positioned to Integrate Cuticular Plate Actin and Somatic Tubulin The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2014 | Pubmed ID: 24381291 E2 Enzyme Inhibition by Stabilization of a Low-affinity Interface with Ubiquitin Nature Chemical Biology. Feb, 2014 | Pubmed ID: 24316736 Bacterial Social Networks: Structure and Composition of Myxococcus Xanthus Outer Membrane Vesicle Chains Environmental Microbiology. Feb, 2014 | Pubmed ID: 23848955 CSBB-ConeExclusion, Adapting Structure Based Solution Virtual Screening to Libraries on Solid Support Journal of Chemical Information and Modeling. Dec, 2013 | Pubmed ID: 24266324 Community Dynamics of Cellulose-adapted Thermophilic Bacterial Consortia Environmental Microbiology. Sep, 2013 | Pubmed ID: 23763762 Three Novel Rice Genes Closely Related to the Arabidopsis IRX9, IRX9L, and IRX14 Genes and Their Roles in Xylan Biosynthesis Frontiers in Plant Science. 2013 | Pubmed ID: 23596448 Isolation and Culture of Endothelial Cells, Pericytes and Perivascular Resident Macrophage-like Melanocytes from the Young Mouse Ear Nature Protocols. Apr, 2013 | Pubmed ID: 23493068 Survey of Renewable Chemicals Produced from Lignocellulosic Biomass During Ionic Liquid Pretreatment Biotechnology for Biofuels. 2013 | Pubmed ID: 23356589 Molecular Architecture of the Chick Vestibular Hair Bundle Nature Neuroscience. Mar, 2013 | Pubmed ID: 23334578 The Role of Bacterial Outer Membrane Vesicles for Intra- and Interspecies Delivery Environmental Microbiology. Feb, 2013 | Pubmed ID: 23227894 Engineering Secondary Cell Wall Deposition in Plants Plant Biotechnology Journal. Apr, 2013 | Pubmed ID: 23140549 Correlative Microscopy for Phylogenetic and Ultrastructural Characterization of Microbial Communities Environmental Microbiology Reports. Feb, 2012 | Pubmed ID: 23757227 Towards Mimicking Short Linear Peptide Motifs: Identification of New Mixed α,β-peptidomimetic Ligands for SLAM-Associated Protein (SAP) by Confocal On-bead Screening Journal of Chemical Biology. Apr, 2012 | Pubmed ID: 23284589 Engineering of Plants with Improved Properties As Biofuels Feedstocks by Vessel-specific Complementation of Xylan Biosynthesis Mutants Biotechnology for Biofuels. 2012 | Pubmed ID: 23181474 Inactivation of OsIRX10 Leads to Decreased Xylan Content in Ricestem Cell Walls and Improved Biomass Saccharification Molecular Plant. Nov, 2012 | Pubmed ID: 23180670 Understanding Changes in Lignin of Panicum Virgatum and Eucalyptus Globulus As a Function of Ionic Liquid Pretreatment Bioresource Technology. Dec, 2012 | Pubmed ID: 23073103 Ablation of the Regulatory IE1 Protein of Murine Cytomegalovirus Alters in Vivo Pro-inflammatory TNF-alpha Production During Acute Infection PLoS Pathogens. 2012 | Pubmed ID: 22952450 On-bead Screens Sample Narrower Affinity Ranges of Protein-ligand Interactions Compared to Equivalent Solution Assays Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Oct, 2012 | Pubmed ID: 22829563 Perivascular-resident Macrophage-like Melanocytes in the Inner Ear Are Essential for the Integrity of the Intrastrial Fluid-blood Barrier Proceedings of the National Academy of Sciences of the United States of America. Jun, 2012 | Pubmed ID: 22689949 Deep-sea Bacteria Enriched by Oil and Dispersant from the Deepwater Horizon Spill Environmental Microbiology. Sep, 2012 | Pubmed ID: 22616650 Isolation and Proteomic Characterization of the Arabidopsis Golgi Defines Functional and Novel Components Involved in Plant Cell Wall Biosynthesis Plant Physiology. May, 2012 | Pubmed ID: 22430844 Loss of Cellulose Synthase-like F6 Function Affects Mixed-linkage Glucan Deposition, Cell Wall Mechanical Properties, and Defense Responses in Vegetative Tissues of Rice Plant Physiology. May, 2012 | Pubmed ID: 22388489 Mammary Collective Cell Migration Involves Transient Loss of Epithelial Features and Individual Cell Migration Within the Epithelium Journal of Cell Science. Jun, 2012 | Pubmed ID: 22344263 Surface Multiheme C-type Cytochromes from Thermincola Potens and Implications for Respiratory Metal Reduction by Gram-positive Bacteria Proceedings of the National Academy of Sciences of the United States of America. Jan, 2012 | Pubmed ID: 22307634 The Cooperative Activities of CSLD2, CSLD3, and CSLD5 Are Required for Normal Arabidopsis Development Molecular Plant. Nov, 2011 | Pubmed ID: 21471331 A Direct Way of Redox Sensing RNA Biology. Jan-Feb, 2011 | Pubmed ID: 21220941 High-throughput Enzymatic Hydrolysis of Lignocellulosic Biomass Via In-situ Regeneration Bioresource Technology. Jan, 2011 | Pubmed ID: 20884206 Deep-sea Oil Plume Enriches Indigenous Oil-degrading Bacteria Science (New York, N.Y.). Oct, 2010 | Pubmed ID: 20736401 Analysis of Protein-small Molecule Interactions by Microscale Equilibrium Dialysis and Its Application As a Secondary Confirmation Method for On-bead Screening Journal of Combinatorial Chemistry. Sep, 2010 | Pubmed ID: 20681515 Identification of a Small Molecule Inhibitor of Importin β Mediated Nuclear Import by Confocal On-bead Screening of Tagged One-bead One-compound Libraries ACS Chemical Biology. Oct, 2010 | Pubmed ID: 20677820 Biofilms: Structures That May Facilitate Cell-cell Interactions The ISME Journal. Sep, 2010 | Pubmed ID: 20631806 The X-ray Crystal Structure of the First RNA Recognition Motif and Site-directed Mutagenesis Suggest a Possible HuR Redox Sensing Mechanism Journal of Molecular Biology. Apr, 2010 | Pubmed ID: 20219472 Comparison of Dilute Acid and Ionic Liquid Pretreatment of Switchgrass: Biomass Recalcitrance, Delignification and Enzymatic Saccharification Bioresource Technology. Jul, 2010 | Pubmed ID: 19945861 Plant Cell Walls Throughout Evolution: Towards a Molecular Understanding of Their Design Principles Journal of Experimental Botany. 2009 | Pubmed ID: 19687127 Mutations in UDP-Glucose:sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds Plant Physiology. Sep, 2009 | Pubmed ID: 19641030 Single Bead Labeling Method for Combining Confocal Fluorescence On-bead Screening and Solution Validation of Tagged One-bead One-compound Libraries Chemistry & Biology. Jul, 2009 | Pubmed ID: 19635409 Confocal Nanoscanning, Bead Picking (CONA): PickoScreen Microscopes for Automated and Quantitative Screening of One-bead One-compound Libraries Journal of Combinatorial Chemistry. Sep-Oct, 2009 | Pubmed ID: 19603813 A Highly Potent and Cellularly Active Beta-peptidic Inhibitor of the P53/hDM2 Interaction Chembiochem : a European Journal of Chemical Biology. Apr, 2009 | Pubmed ID: 19267375 Three-dimensional Macromolecular Organization of Cryofixed Myxococcus Xanthus Biofilms As Revealed by Electron Microscopic Tomography Journal of Bacteriology. Apr, 2009 | Pubmed ID: 19168614 Electron Tomography, Three-dimensional Fourier Analysis and Colour Prediction of a Three-dimensional Amorphous Biophotonic Nanostructure Journal of the Royal Society, Interface / the Royal Society. Apr, 2009 | Pubmed ID: 19158016 Terminal Adenosyl Transferase Activity of Posttranscriptional Regulator HuR Revealed by Confocal On-bead Screening Journal of Molecular Biology. Feb, 2009 | Pubmed ID: 19109971 Natural Acidophilic Biofilm Communities Reflect Distinct Organismal and Functional Organization The ISME Journal. Feb, 2009 | Pubmed ID: 18843299 Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation Annals of the New York Academy of Sciences. 2008 | Pubmed ID: 18596327 Covalent Fluorescence Labeling of His-tagged Proteins on the Surface of Living Cells Chembiochem : a European Journal of Chemical Biology. Jun, 2008 | Pubmed ID: 18461582 Three-dimensional Architecture of Hair-bundle Linkages Revealed by Electron-microscopic Tomography Journal of the Association for Research in Otolaryngology : JARO. Jun, 2008 | Pubmed ID: 18421501 Electron Tomography: a 3D View of the Subcellular World Analytical Chemistry. Nov, 2007 | Pubmed ID: 18044021 Genetic Evidence for Three Unique Components in Primary Cell-wall Cellulose Synthase Complexes in Arabidopsis Proceedings of the National Academy of Sciences of the United States of America. Sep, 2007 | Pubmed ID: 17878302 Identification and Mechanistic Characterization of Low-molecular-weight Inhibitors for HuR Nature Chemical Biology. Aug, 2007 | Pubmed ID: 17632515 Pseudomonas Aeruginosa Forms Biofilms in Acute Infection Independent of Cell-to-cell Signaling Infection and Immunity. Aug, 2007 | Pubmed ID: 17562773 Beyond Dimerization: a Membrane-dependent Activation Model for Interleukin-4 Receptor-mediated Signalling Journal of Molecular Biology. Mar, 2007 | Pubmed ID: 17223132 High-pressure Freezing, Cellular Tomography, and Structural Cell Biology BioTechniques. Aug, 2006 | Pubmed ID: 16925014 Structural and Biophysical Characterization of the EphB4*ephrinB2 Protein-protein Interaction and Receptor Specificity The Journal of Biological Chemistry. Sep, 2006 | Pubmed ID: 16867992 Multi-photon Excitation of Intrinsic Protein Fluorescence and Its Application to Pharmaceutical Drug Screening Assay and Drug Development Technologies. Apr, 2005 | Pubmed ID: 15871690 The Effect of RNA Secondary Structures on RNA-ligand Binding and the Modifier RNA Mechanism: a Quantitative Model Gene. Jan, 2005 | Pubmed ID: 15716109 MRNA Openers and Closers: Modulating AU-rich Element-controlled MRNA Stability by a Molecular Switch in MRNA Secondary Structure Chembiochem : a European Journal of Chemical Biology. Oct, 2004 | Pubmed ID: 15457527 The Chemical Hunt for the Identification of Drugable Targets Current Opinion in Chemical Biology. Aug, 2004 | Pubmed ID: 15338571 Confocal Fluorescence Detection Expanded to UV Excitation: the First Continuous Fluorimetric Assay of Human Steroid Sulfatase in Nanoliter Volume Assay and Drug Development Technologies. Feb, 2004 | Pubmed ID: 15090207 Volumetric Feature Extraction and Visualization of Tomographic Molecular Imaging Journal of Structural Biology. Oct-Nov, 2003 | Pubmed ID: 14643216 Three-dimensional Crystallization of the Escherichia Coli Glycerol-3-phosphate Transporter: a Member of the Major Facilitator Superfamily Protein Science : a Publication of the Protein Society. Dec, 2003 | Pubmed ID: 14627735 Structure and Mechanism of the Glycerol-3-phosphate Transporter from Escherichia Coli Science (New York, N.Y.). Aug, 2003 | Pubmed ID: 12893936 Fluorescent Fingerprinting of Molecular Recognition Landscapes J.J.L. Thanks Wolfgang Rettig (Humboldt University) for His Generosity During the Preparation of This Manuscript and the Uebbing Foundation for Support. M.A. Thanks Jan E. De Vries for Continuous Support and Encouragement Angewandte Chemie (International Ed. in English). May, 2001 | Pubmed ID: 11385663 Simplified Volumetric Models as an Effective Strategy for Segmenting Actin Networks in Cryo-Electron Tomograms Junha Song1, Manfred Auer1 1Department of Biomedical Engineering, School of Biological Sciences and Medical Engineering, Southeast University JoVE 64845 Neuroscience From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data Wen-Ting Tsai1, Ahmed Hassan1, Purbasha Sarkar2, Joaquin Correa1,3, Zoltan Metlagel1, Danielle M. Jorgens1, Manfred Auer1,2 1Life Sciences Division, Lawrence Berkeley National Laboratory, 2Joint Bioenergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, 3National Energy Research Scientific Computing Center, Lawrence Berkeley National Laboratory JoVE 51673 Bioengineering
Simplified Volumetric Models as an Effective Strategy for Segmenting Actin Networks in Cryo-Electron Tomograms Junha Song1, Manfred Auer1 1Department of Biomedical Engineering, School of Biological Sciences and Medical Engineering, Southeast University JoVE 64845 Neuroscience
From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data Wen-Ting Tsai1, Ahmed Hassan1, Purbasha Sarkar2, Joaquin Correa1,3, Zoltan Metlagel1, Danielle M. Jorgens1, Manfred Auer1,2 1Life Sciences Division, Lawrence Berkeley National Laboratory, 2Joint Bioenergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, 3National Energy Research Scientific Computing Center, Lawrence Berkeley National Laboratory JoVE 51673 Bioengineering
