Richard D. Leapman Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering National Institutes of Health Biography Publications Institution JoVE Articles Richard D. Leapman has not added a biography. If you are Richard D. Leapman and would like to personalize this page please email our Author Liaison for assistance. Publications Tethered Platelet Capture Provides a Mechanism for Restricting Circulating Platelet Activation to the Wound Site Research and Practice in Thrombosis and Haemostasis. Feb, 2023 | Pubmed ID: 36865905 Palmitoylation of A-kinase Anchoring Protein 79/150 Modulates Its Nanoscale Organization, Trafficking, and Mobility in Postsynaptic Spines Frontiers in Synaptic Neuroscience. 2022 | Pubmed ID: 36186623 Venous Puncture Wound Hemostasis Results in a Vaulted Thrombus Structured by Locally Nucleated Platelet Aggregates Communications Biology. Sep, 2021 | Pubmed ID: 34531522 Circadian Modulation of Neurons and Astrocytes Controls Synaptic Plasticity in Hippocampal Area CA1 Cell Reports. Oct, 2020 | Pubmed ID: 33053337 Determination of Secretory Granule Maturation Times in Pancreatic Islet β-cells by Serial Block-face Electron Microscopy Journal of Structural Biology. Oct, 2020 | Pubmed ID: 32736074 Quantitative Method for Estimating Stain Density in Electron Microscopy of Conventionally Prepared Biological Specimens Journal of Microscopy. Feb, 2020 | Pubmed ID: 31994199 Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography Frontiers in Neuroanatomy. 2018 | Pubmed ID: 30581381 SNARE-dependent Membrane Fusion Initiates α-granule Matrix Decondensation in Mouse Platelets Blood Advances. Nov, 2018 | Pubmed ID: 30401752 Biological Serial Block Face Scanning Electron Microscopy at Improved Z-resolution Based on Monte Carlo Model Scientific Reports. Aug, 2018 | Pubmed ID: 30154532 Dotted Core-Shell Nanoparticles for T -Weighted MRI of Tumors Advanced Materials (Deerfield Beach, Fla.). Jul, 2018 | Pubmed ID: 29972604 Comparison of 3D Cellular Imaging Techniques Based on Scanned Electron Probes: Serial Block Face SEM Vs. Axial Bright-field STEM Tomography Journal of Structural Biology. Jun, 2018 | Pubmed ID: 29408702 Cells Containing Aragonite Crystals Mediate Responses to Gravity in Trichoplax Adhaerens (Placozoa), an Animal Lacking Neurons and Synapses PloS One. 2018 | Pubmed ID: 29342202 Application of EELS and EFTEM to the Life Sciences Enabled by the Contributions of Ondrej Krivanek Ultramicroscopy. Sep, 2017 | Pubmed ID: 28258873 PAR1 Activation Induces Rapid Changes in Glutamate Uptake and Astrocyte Morphology Scientific Reports. Mar, 2017 | Pubmed ID: 28256580 Tri-stimuli-responsive Biodegradable Theranostics for Mild Hyperthermia Enhanced Chemotherapy Biomaterials. May, 2017 | Pubmed ID: 28254692 Golgi Proteins in Circulating Human Platelets Are Distributed Across Non-stacked, Scattered Structures Platelets. Jun, 2017 | Pubmed ID: 27753523 Physicochemical Characterization of Ferumoxytol, Heparin and Protamine Nanocomplexes for Improved Magnetic Labeling of Stem Cells Nanomedicine : Nanotechnology, Biology, and Medicine. Feb, 2017 | Pubmed ID: 27520728 Compressed Sensing Electron Tomography for Determining Biological Structure Scientific Reports. Jun, 2016 | Pubmed ID: 27291259 Biointeractions of Ultrasmall Glutathione-coated Gold Nanoparticles: Effect of Small Size Variations Nanoscale. Mar, 2016 | Pubmed ID: 26934984 STEM Tomography Reveals That the Canalicular System and α-granules Remain Separate Compartments During Early Secretion Stages in Blood Platelets Journal of Thrombosis and Haemostasis : JTH. Mar, 2016 | Pubmed ID: 26663480 PSD-95 Family MAGUKs Are Essential for Anchoring AMPA and NMDA Receptor Complexes at the Postsynaptic Density Proceedings of the National Academy of Sciences of the United States of America. Dec, 2015 | Pubmed ID: 26604311 Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States PloS One. 2015 | Pubmed ID: 26565809 Self-organized Mn-Block Copolymer Complexes and Their Use for MR Imaging of Biological Processes Journal of Materials Chemistry. B. 2014 | Pubmed ID: 25364506 Passive Diffusion As a Mechanism Underlying Ribbon Synapse Vesicle Release and Resupply The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jul, 2014 | Pubmed ID: 24990916 Targeted Therapeutic Nanotubes Influence the Viscoelasticity of Cancer Cells to Overcome Drug Resistance ACS Nano. May, 2014 | Pubmed ID: 24708375 A Structural Framework for a Near-minimal Form of Life: Mass and Compositional Analysis of the Helical Mollicute Spiroplasma Melliferum BC3 PloS One. 2014 | Pubmed ID: 24586297 Biodegradable Gold Nanovesicles with an Ultrastrong Plasmonic Coupling Effect for Photoacoustic Imaging and Photothermal Therapy Angewandte Chemie (International Ed. in English). Dec, 2013 | Pubmed ID: 24318645 Cryo-electron Tomography of the Magnetotactic Vibrio Magnetovibrio Blakemorei: Insights into the Biomineralization of Prismatic Magnetosomes Journal of Structural Biology. Feb, 2013 | Pubmed ID: 23246783 Elemental Mapping by Electron Energy Loss Spectroscopy in Biology Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23086878 Mass Mapping of Amyloid Fibrils in the Electron Microscope Using STEM Imaging Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23086877 Aggregation and Fibril Morphology of the Arctic Mutation of Alzheimer's Aβ Peptide by CD, TEM, STEM and in Situ AFM Journal of Structural Biology. Oct, 2012 | Pubmed ID: 22750418 Development and Application of STEM for the Biological Sciences Ultramicroscopy. Dec, 2012 | Pubmed ID: 22749213 Direct Visualization of CaMKII at Postsynaptic Densities by Electron Microscopy Tomography The Journal of Comparative Neurology. Dec, 2012 | Pubmed ID: 22627922 Nuclear Mapping of Nanodrug Delivery Systems in Dynamic Cellular Environments ACS Nano. Jun, 2012 | Pubmed ID: 22540867 Synthesis, Characterization, and Direct Intracellular Imaging of Ultrasmall and Uniform Glutathione-coated Gold Nanoparticles Small (Weinheim an Der Bergstrasse, Germany). Apr, 2012 | Pubmed ID: 22517616 Polyaspartic Acid Coated Manganese Oxide Nanoparticles for Efficient Liver MRI Nanoscale. Dec, 2011 | Pubmed ID: 22064945 Combining Portable Raman Probes with Nanotubes for Theranostic Applications Theranostics. 2011 | Pubmed ID: 21769298 PSD-95 is Required to Sustain the Molecular Organization of the Postsynaptic Density The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Apr, 2011 | Pubmed ID: 21525273 Dual-axis Electron Tomography of Biological Specimens: Extending the Limits of Specimen Thickness with Bright-field STEM Imaging Journal of Structural Biology. Apr, 2011 | Pubmed ID: 21055473 Distribution and Clearance of PEG-single-walled Carbon Nanotube Cancer Drug Delivery Vehicles in Mice Nanomedicine (London, England). Dec, 2010 | Pubmed ID: 21143032 Layer-specific Variation of Iron Content in Cerebral Cortex As a Source of MRI Contrast Proceedings of the National Academy of Sciences of the United States of America. Feb, 2010 | Pubmed ID: 20133720 Imaging the Distribution of Individual Platinum-based Anticancer Drug Molecules Attached to Single-wall Carbon Nanotubes Nanomedicine (London, England). Oct, 2009 | Pubmed ID: 19839812 Nanoscale 3D Cellular Imaging by Axial Scanning Transmission Electron Tomography Nature Methods. Oct, 2009 | Pubmed ID: 19718033 Influence of Melanosome Dynamics on Melanoma Drug Sensitivity Journal of the National Cancer Institute. Sep, 2009 | Pubmed ID: 19704071 Physiologic Upper Limit of Pore Size in the Blood-tumor Barrier of Malignant Solid Tumors Journal of Translational Medicine. Jun, 2009 | Pubmed ID: 19549317 Seeded Growth of Beta-amyloid Fibrils from Alzheimer's Brain-derived Fibrils Produces a Distinct Fibril Structure Proceedings of the National Academy of Sciences of the United States of America. May, 2009 | Pubmed ID: 19376973 Targeted Killing of Cancer Cells in Vivo and in Vitro with EGF-directed Carbon Nanotube-based Drug Delivery ACS Nano. Feb, 2009 | Pubmed ID: 19236065 Effective Transvascular Delivery of Nanoparticles Across the Blood-brain Tumor Barrier into Malignant Glioma Cells Journal of Translational Medicine. Dec, 2008 | Pubmed ID: 19094226 Molecular Structural Basis for Polymorphism in Alzheimer's Beta-amyloid Fibrils Proceedings of the National Academy of Sciences of the United States of America. Nov, 2008 | Pubmed ID: 19015532 Organization of the Core Structure of the Postsynaptic Density Proceedings of the National Academy of Sciences of the United States of America. Mar, 2008 | Pubmed ID: 18326622 RESP18, a Homolog of the Luminal Domain IA-2, is Found in Dense Core Vesicles in Pancreatic Islet Cells and is Induced by High Glucose The Journal of Endocrinology. Nov, 2007 | Pubmed ID: 17951542 Protein Oxidation Implicated As the Primary Determinant of Bacterial Radioresistance PLoS Biology. Apr, 2007 | Pubmed ID: 17373858 Localizing Specific Elements Bound to Macromolecules by EFTEM Methods in Cell Biology. 2007 | Pubmed ID: 17327176 Evaluation of Bioreactor-cultivated Bone by Magnetic Resonance Microscopy and FTIR Microspectroscopy Bone. Apr, 2007 | Pubmed ID: 17174620 Melanosomal Sequestration of Cytotoxic Drugs Contributes to the Intractability of Malignant Melanomas Proceedings of the National Academy of Sciences of the United States of America. Jun, 2006 | Pubmed ID: 16777967 Mass of the Postsynaptic Density and Enumeration of Three Key Molecules Proceedings of the National Academy of Sciences of the United States of America. Aug, 2005 | Pubmed ID: 16061821 Self-propagating, Molecular-level Polymorphism in Alzheimer's Beta-amyloid Fibrils Science (New York, N.Y.). Jan, 2005 | Pubmed ID: 15653506 Novel Techniques in Electron Microscopy Current Opinion in Neurobiology. Oct, 2004 | Pubmed ID: 15464893 Organization of Designed Nanofibrils Assembled from Alpha-helical Peptides As Determined by Electron Microscopy Journal of Peptide Science : an Official Publication of the European Peptide Society. May, 2004 | Pubmed ID: 15160841 Promoting Trimerization of Soluble Human Immunodeficiency Virus Type 1 (HIV-1) Env Through the Use of HIV-1/simian Immunodeficiency Virus Chimeras Journal of Virology. Mar, 2004 | Pubmed ID: 14963123 Mass Distribution and Spatial Organization of the Linear Bacterial Motor of Spiroplasma Citri R8A2 Journal of Bacteriology. Mar, 2003 | Pubmed ID: 12618463 A Structural Model for Alzheimer's Beta -amyloid Fibrils Based on Experimental Constraints from Solid State NMR Proceedings of the National Academy of Sciences of the United States of America. Dec, 2002 | Pubmed ID: 12481027 Oligomeric Structure of the Human Immunodeficiency Virus Type 1 Envelope Protein on the Virion Surface Journal of Virology. Aug, 2002 | Pubmed ID: 12097599 Oligomeric Structure of Virion-associated and Soluble Forms of the Simian Immunodeficiency Virus Envelope Protein in the Prefusion Activated Conformation Proceedings of the National Academy of Sciences of the United States of America. Dec, 2001 | Pubmed ID: 11752436 Multiple Modes of Calcium-induced Calcium Release in Sympathetic Neurons II: a [Ca2+](i)- and Location-dependent Transition from Endoplasmic Reticulum Ca Accumulation to Net Ca Release The Journal of General Physiology. Jul, 2001 | Pubmed ID: 11429447 Multiple Quantum Solid-state NMR Indicates a Parallel, Not Antiparallel, Organization of Beta-sheets in Alzheimer's Beta-amyloid Fibrils Proceedings of the National Academy of Sciences of the United States of America. Nov, 2000 | Pubmed ID: 11069287 Stimulus-secretion Coupling in Neurohypophysial Nerve Endings: a Role for Intravesicular Sodium? Proceedings of the National Academy of Sciences of the United States of America. Mar, 1999 | Pubmed ID: 10077662 Activity-dependent Calcium Sequestration in Dendrites of Hippocampal Neurons in Brain Slices The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 1997 | Pubmed ID: 9348342 Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis Smita Joshi*1, Alexis N. Smith1, Kanakanagavalli Shravani Prakhya1, Hammodah R. Alfar1, Joshua Lykins1, Ming Zhang1, Irina Pokrovskaya2, Maria Aronova3, Richard D. Leapman3, Brian Storrie2, Sidney W. Whiteheart*1 1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, 3Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health JoVE 64985 Medicine
Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis Smita Joshi*1, Alexis N. Smith1, Kanakanagavalli Shravani Prakhya1, Hammodah R. Alfar1, Joshua Lykins1, Ming Zhang1, Irina Pokrovskaya2, Maria Aronova3, Richard D. Leapman3, Brian Storrie2, Sidney W. Whiteheart*1 1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, 3Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health JoVE 64985 Medicine