Enhancing Protein Visualization with Antibody-Mediated Live Imaging

January 19th, 2024

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Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan^2,3, Huapeng H. Yu¹

¹School of Life Sciences, SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science, Hong Kong University of Science and Technology

* These authors contributed equally

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Dieses Protokoll beschreibt die maßgeschneiderte antikörperbasierte Fluoreszenzmarkierung und Injektion in frühe Drosophila-Embryonen , um Live-Imaging von Proteinen mit geringer Häufigkeit oder posttranslationalen Modifikationen zu ermöglichen, die mit herkömmlichen GFP/mCherry-Tag-Ansätzen nur schwer zu erkennen sind.

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Enhancing Protein Visualization with Antibody-Mediated Live Imaging

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