Methylated DNA Immunoprecipitation Kelsie L. Thu1, 2, 3, Emily A. Vucic1, 2, 4, Jennifer Y. Kennett2, 4, Cameron Heryet5, Carolyn J. Brown6, Wan L. Lam4, 3, 2, Ian M. Wilson2, 4
1These authors contributed equally, 2Department of Cancer Genetics and Developmental Biology, BC Cancer Research Centre, 3Interdisciplinary Oncology Program, University of British Columbia, 4Department of Pathology and Laboratory Medicine, University of British Columbia, 5Photography/Video Production, Multi-Media Services, BC Cancer Agency, 6Department of Medical Genetics, Life Sciences Institute, University of British Columbia
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| This video demonstrates the protocol for methylated DNA immunoprecipitation (MeDIP). MeDIP is a two day procedure that selectively extracts methylated DNA fragments from a genomic DNA sample using antibodies with specificity for 5 -methylcytosine (anti-5 mC). |
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Elevated Plus Maze for Mice Munekazu Komada1, Keizo Takao2, Tsuyoshi Miyakawa2
1Genetic Engineering and Functional Genomics Unit, Kyoto University Graduate School of Medicine, 2Institute for Comprehensive Medical Science Division of Systems Medicine, Fujita Health University
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| The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior in mice. Here, we present a movie showing the detailed procedures for conducting the test. |
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Electrochemotherapy of Tumours Gregor Sersa1, Damijan Miklavcic2
1Department of Experimental Oncology, Institute of Oncology Ljubljana, 2Faculty of Electrical Engineering, University of Ljubljana
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| Electrochemotherapy is a combined use of certain chemotherapeutic drugs such as cisplatin and bleomycin and electric pulses applied to the treated tumour nodule. We present the clinical protocol of electrochemotherapy for treatmen of subcutaneous and cutaneous metastases of melanoma. |
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